Effect of Bioresonance Therapy on Antioxidant System in Lymphocytes in Patients with Rheumatoid Arthritis

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B. I, Islamov, R. M. Balabanova, V. A. untikov, Yu. V. Gotovskii, and E. E. Meizerov”

Translated from Byulleten Experimental noi Biologii Meditsiny, Vol. 134, No. 9. pp. 237-190, September, 2002 Original article submitted January 24, 2002


We measured activities of superoxide dismutase, catalase, and glutathione peroxidase and content of nonprotein thiol groups (reduced glutathione) in blood lymphocytes from patients with rheumatoid arthritis before and during bioresonance therapy. The state of the antioxi­dant system in lymphocyte from patients receiving standard pharmacotherapy was characteri­zed by activation of the key antioxidant enzymes and decreased content of thiol groups. Bioresonance therapy increased the content of thiol groups and normalized activities of su­peroxide dismutase and glutathione peroxidase. However, catalase activity remained above the control. Changes in the lymphocyte antioxidant system indicate that bioresonance thera­py activates nonspecific protective mechanisms in patients with rheumatoid arthritis.

The role of reactive oxygen species in the pathogene­sis of autoimmune disorders, including rheumatoid arthritis (RA) attracts much attention [51. Reactive oxygen species (superoxide radical, hydroxyl radical,

peroxynitrite, etc.) are involved in the formation of tissue damages during RA 19]. Little is known about *e antioxidant system of peripheral blood lymphocy­.es in patients with RA, It remains unclear whether bio­resonance therapy (BRT) can regulate this system [2],

Here we studied the lymphocyte antioxidant sys­tem in patients with RA and evaluated the possibility of correcting disturbances by means of BRT.

MATERIALS AND METHODS

We examined 20 women (19-60 years) with RA of stages II-III. All patients received nonsteroid anti-rheumatic drug diclofenac. (daily dose 50-200 mg.), 7 patients received prednisolorte (?1,-.-7. daily dose 5-15mg) and one of basic preparations (aminoquinoline preparations, methotrexate. and sulfasalazine). BRT was performed on an NEDIS- OLL complex once a week (20-30 min) for 9 months_ Electronic analogues of homeopathic preparations were selected individually (7)

Blood samples were taken before, by the end, and 2-3 months after the therapy.

The control group included 10 heathy women of the same age.

Lymphocytes (mononuclear cells) were obtained by centrifugation of the peripheral blood in a Vero-gratin- ico€1 density gradient (p=2.077) and washed 2 times in phosphate buffer. Activities of antioxidant enzymes were measured in special buffers. The cells were destroyed by repeated freezing and thawing (.-20-‘C). Activities of antioxidant enzymes were mea­sured.

The concentration of reduced thiol groups was measured using 5,5′-clithio-bis(2-nitrobenzoic) acid as described elsewhere [4]. The reaction between 5,5′- dithio-bis(2-nitrobenzoic) acid and acid-soluble SH­groups yields a colored product with an absorption maximum at λ=412 nm.

Glutathione peroxidase (GSH-Px) activity was estimated by utilization of reduced glutathione usirw tert-butyl hydroperoxide as the substrate 14]. Total superoxide dismutase (SOD) activity was measured as described elsewhere (10). Catalase activity was de­termined by the rate of H2O2, degradation [12].

The results were analyzed by nonparametric Van der Warden X test. The differences were significant at p<0.05.

RESULTS

Before BRT, activities of SOD, catalase, and GSH-Px in blood lymphocytes from RA patients surpassed the normal by 109, 42, and 92.•0, respectively. The content of nonprotein thiol groups in these patients decreased 1.y 17% compared to the control ( 12. 1). More than 90% thiol groups were presented by glutathione. Du­ring BRT activity of GSH-Px decreased by 37%, but remained 41% above the control. SOD activity de­creased by 19% and was 169% of the control. Catalase activity remained unchanged. The content of nonpro­tein thiol groups increased by 44% and reached 1)1% of the control level ( ig. I ).

These changes in the content of nonprotein thiol croups and activities of antioxidant enzymes persisted after termination of BRT. The content of nonprotein groups increased by 67% and was 135% of the control. GSH-Px activity approached the control level and was 58% lower than that observed before the ther­apy. SOD activity progressively decreased, but re­mained 51% above the control. Catalase activity re­mained unchanged.

RA is usually associated with decreased intra­cellular SOD activity [141. Our observations showed that SOD activity in RA patients is significantly higher than in healthy donors. This is probably related to the therapy with nonsteroid antiinflammatory drugs, hor­mones, and immunosuppre.ssive preparations and com­pensatory cell response to continuous production of free oxygen radicals. Unfortunately, most patients re­ceived standard medicinal preparations before and during BRT. In patiens with low SOD activity non-steroid antiinflammatory drugs markedly increase this parameter [ i3J. Previous studies showed that inflam­mation and bacterial phagocyrosis are suppressed by SOD –Lr 1 1 Changes in SOD activity improve the state of patiens(13).

Activities of SOD
Activities of SOD

In our observations clinical state of RA patients improved during BRT despite the decrease in SOD activity_ Ir can be assumed that BRT reduces the in­flammatory response and decreases the strain of adap­tive reactions.

Catalase is a heme-containing enzyme localized in peroxisomes.This enzyme degrades H2O2 which contributes to generation of reactive oxygen meta­bolites, Published data show that 0,5% O2, formed after H2O2 degradation is in activated singlet state [6]. In our experiments catalase activity remained unchand. Probably, BRT did not modulate the formation of 1-1,0,. It cardiot be excluded that catalase is not the key antioxidant enzyme in lymphocytes.

GSH-Px is localized in the cytoplasm and mito­chondria. This enzyme catalyzes degradation of vari­ous peroxides by oxidizing crlutarhione with the for­mation of its conjuzates. GSH-Px affinity for H2O, surpasses that of catalase. Therefore. GSH-Px is more effective at low F.O. concentrations. In our observa­tions GSH-Px activiry in RA patients markedly surpas­sed that in healthy donors. Enzyme activity progressi­vely decreased during BRT and did not differ from the control after the therapy,

SH-containing compounds play an important role in the protection of cells from OH radicals formed in the enton reaction.Thiol compounds maintain oxida­tion-reduction homeostasis in cells and tissues. Stress roduces reversible oxidative modification of thiol ,roups, which serves as the nonspecific reaction to extreme factors [1]. These changes modi-ly the state of cell membranes, :heir permeability, and adhesive pro­perties and affect enzymes activEties and ced prolife­rgion. Previous studies showed that SEI-containing compounds First under2o oxidaton. This prevents other function’, aroups and molecules from oxidation_ Chan­ges in the concentration of nonprotein thiol groups and GSH-Px activity during BRT were inversely related. The ratio between reduced and oxidized SN-groups reflects nonspeciF:c resistance of the organisms [7].

Our observations indicate that before BRT the lymphocyte antioxidant system in RA patients recei­ving standard pharmacotherapy is in the strained state. Activities of key antioxidant enzymes GSH-Px, SOD, and catalase increase. while the content of reduced thiol groups decreases. BRT increases the content of reduced S H-groups and normalizes SOD and GSH-Px activities. The increase in glutathione content and in­duction of stress-protein synthesis improve protective reserves of the organism [3]. These data show that BRT produces a varier; of effects on patients. BRT stimulates nonspecific mechanisms protectmg the organism from damaging exocrenous and endogenous factors.

REFERENCES

  1. M.V. Bilenko, Ischerie and Reperfusion Damages to Organs (in Russian), Moscow (2000)
  2. Bioresonance Therapy (Methodical Recommendations No. 2000/74)(In Russian), Moscow (2000)

And others ….