Bioresonance is a new holistic approach on the human system. The following study will make an attempt to thoroughly exam the overall bioresonance methodology as far as its contribution to dealing with specific diseases, taking into account epidemiological data from various laboratories and medical centers in Greece that apply this technique, while at the same time will study the possibilities of the bioresonance implementation in Laboratory using the sister chromatid exchange methodology (SCEs). It is a specialized technique of controlling mutagenic substances in cellular level by examining distal blood lymphocytes in vitro present or some non mutagens (such as melphalan and irinotecan) and with the simultaneous action of antioxidants (such as vitamins or amifostine), cross checking the possibilities of applying bioresonance in these very studies.
The statistical data process includes the study of several parameters such as age, gender, family status, schooling, occupation, the presence of intolerance or even diseases, as well as the presence and balancing of allergic burdens. 312 patients in total from health clinics and bioresonance centers in Athens, Thessaloniki and Xanthi took part in the process of this study. The data process of these cases will be conducted under the Praxiteles statistics program.
The purpose of this Study is the examination of the effect of bioresonance methodology as an alternative method of cell protection as well as of human protection from harmful factors.
Melphalan and/or irinotecan will be used as genotoxic factors, substances whose genotoxic action is thoroughly described in academic bibliography, whereas melphalan being significantly powerful. It will also be examined in cultivating distal lymphocytes from normal donors or patients with intolerance along with the genotoxics and the antioxidants (such as vitamins E, C, or the amifostine), in order to decrease or even minimize the genotoxicity in human chromosomes caused by mutagens. In order to control the action of these substances with a simultaneous bioresonance application we will be using the Fluoresence Plus Giemsa method. In this way, the chromatid exchanges will be visible with the use of a photonic microscope, and thus we will be able to evaluate the necessary mass of metaphases, so as to draw a safe conclusion, statistically valuable. Following 72 hours of cultivating distal lymphocytes and after the specialized coloring, photo shooting, evaluating and statistical data processing, we will examine in every blood sample two specific parameters, the existence or not of genotoxicity corresponding to the levels of sister chromatid exchanges (SCEs) and the existence or not of cytotoxicity and cytostatic behavior as it appears in the measurement of the cell propagation process (RPIs).